NOS3 and brain ischemia: Since our in vivo experimental data demonstrated that FAD012 exhibited prophylactic protective effects against MCAO-induced cerebral ischemia/reperfusion injuries and, coincidentally, suppressed a decrease in the expression level of the eNOS of the vascular endothelium in the cerebral cortex (Figure 3, Figure 4, Figure 5 and Figure 6 and Table 2), RBMVEC was exploited to investigate the effects of FAD012 or FA on the expression level of eNOS in cells following oxidative stress stimulation.