In order to evaluate the performance of the NTA in different experimental models, EVs size and concentration were measured in the sera of human patients with NAFLD, AIH, and healthy donors, in sera isolated from young and aged rats, and in the sera obtained from Gunn rats with homozygote Ugt1a mutation and control rats (Figure 1, Representative NTA images are shown in Supplementary Figure S3). This evidence concerns the gene UGT1A1 and autoimmune hepatitis.