Because ovarian cancer showed the highest gene amplification frequency of TONSL, and the gene expression was associated with poor prognosis, we examined whether loss of TONSL changes cancer cell behavior using ovarian serous adenocarcinoma cell lines (Figure 4A) transduced by shTONSL- or shNC-expressing lentivirus. The gene discussed is TONSL; the disease is ovarian serous adenocarcinoma.