Although we found that galectin-3 resided in NETs while neutrophils underwent NETosis (Figure 3A), further experiments would be required to prove galectin-3 as an autoantigen residing in NETs, such as isolating galectin-3 from NETs and then performing anti-galectin-3 Ab ELISA as depicted in Figure 1C. Second, a contradictory scenario in Gal-3 KO lupus mice has been reported by Beccaria and colleagues [36]. The gene discussed is LGALS3; the disease is systemic lupus erythematosus.