In three-month-old male and female 3xTg-AD mice that harbor mutations in the APP, PSEN1, and tau genes, chronic voluntary alcohol consumption (25% w/v alcohol + 0.1% saccharin w/v in water) for four months increased the Aβ (42/40) ratio and total tau protein in the lateral entorhinal cortex (LEC) and prefrontal cortex (PFC), which is consistent with enhanced AD-related pathological changes. The gene discussed is PSEN1; the disease is Alzheimer disease.