Alcohol treatment increased the expression of: APP, APP-CTFs, and BACE-1 in 2EB2 cells, Aβ40 and Aβ42 levels in 20E2 cell lysates, and APP and BACE-1 in SH105 and SH-SY5Y neuronal cell lines.Conclusion: Alcohol exposure promotes APP processing and aggravates AD-associated phenotypes in a dose-dependent manner. The gene discussed is BACE1; the disease is Alzheimer disease.