Our results show that C9 and CsA potently suppressed the expression of the CSC markers, including integrin α6, CD133, CD44, ALDH1A1, Nanog, Oct4, and Sox2, by downregulating CypA/CD147 expression and EGFR activity in EGFR-mutant NSCLC CSCs. The gene discussed is PROM1; the disease is non-small cell lung carcinoma.