As shown in Supplementary Figure S3, the behavioral indexes were significantly positively correlated with the PSD95 puncta density (OFT: p = 0.0004, r = 0.8525; SPT: p = 0.0240, r = 0.6433) and the microglial territory area (OFT: p = 0.0190, r = 0.6626; SPT: p = 0.0007, r = 0.8354) but negatively correlated with the PSD95 IntDen per microglia (OFT: p = 0.0210, r = −0.6524) in the IL, indicating that the anxiety- and depression-like behavior induced by adolescent HFD consumption was closely related to neuroplasticity, microglial morphology, and the microglial PSD95+ inclusions in the mPFC. This evidence concerns the gene DLG4 and major depressive disorder.