In this study, we utilized the interaction system of FKBP12 with FRB as a PPI system, rapamycin as a compound, and HEK-293 cells and COS-7 cells as donor cells, and finally refractory cancer cells such as A549, MDA-MB-231, and Panc-1 as recipient cells. This evidence concerns the gene FKBP1A and cancer.