GPT and hydrops fetalis: Consistent with this hypothesis, a previously performed analysis of plasma biomarkers, including alanine aminotransferase (ALT), aspartate aminotransferase (AST) and cholesterol [4,5], showed a significant ALT increase in HF and, to a lesser extent, in LF-HC mice compared to SD-fed animals after 12 months (p = 0.002), while there were no differences in ALT, AST and cholesterol levels among the three groups after 18 months (Figure 1e).