This includes measuring free cortisol in 24-h urine, basal levels of adrenocorticotropic hormone (ACTH), dehydroepiandrosterone sulfate (DHEA-S), 17-OH progesterone (17HP), androstenedione, and testosterone (only in women), as well as 17-beta-estradiol (only in men and postmenopausal women), 11-deoxycortisol (if available), potassium, and aldosterone/renin ratio (for patients with hypertension or unexplained hypokalaemia). This evidence concerns the gene POMC and Hypokalemia.