In fact, the permeability of HCMEC/D3 cells has been reported to be increased in stress conditions and pro-inflammatory cytokines and chemokines such as TNFα and CCL2 via signaling pathways as JNK, PKC or NFκB, which could be included in the EVs produced by tumor cell lines [40,41]. The gene discussed is PRRT2; the disease is neoplasm.