Regarding non-DNA binding factors, at least three are known to be directly involved in the regulation of transcription; SUB1 (required for osteosarcoma cell proliferation) and EDF1 (the upper aerodigestive tract) are transcriptional coactivators, whereas SPTY2D1 (in the cervix) is a histone chaperone. This evidence concerns the gene SUB1 and osteosarcoma.