H-Tau.K257T/P301S mice (9 weeks of age) were anesthetized by Isoflurane, and stereotactically injected with human pathological Tau mix from human AD brains (hippocampus 2.5 μg + overlaying cortex 1 μg) in PBS (total of 3.5 μg of pTau per brain). Here, MAPT is linked to Alzheimer disease.