Moreover, FN1, COL1, and iNOS axis-mediated liver fibrosis were caused by DOX, and our data documented the fibrotic effect of DOX via the upregulation of VEGF, Wnt7b, β-catenin, FN1, Col-1, TGFβ1, iNos, and Bax in the DOX group. The gene discussed is TGFB1; the disease is Hepatic fibrosis.