By applying our primer sets for the promoter–exon 1 region and the intron 1 regions A and B (Figure 1) to six tumor tissue (luminal A: T7, T8; luminal B: T10, T17; Her2-positive: T18; triple-negative: T14) and four control tissue samples (C1–C4), all CpGs showed methylation levels <15% (Figure 2b). This evidence concerns the gene ERBB2 and neoplasm.