TOPBP1 and small cell osteogenic sarcoma: Quantitative real-time PCR revealed that BRCA1, RAD51, BIRC5, CDC6, E2F1, MCM4, CDC20, TUBA4A, TUBG1, CCNA1, CCNB1, CCNA2, CHEK1, TOPBP1, MAD2L1, CDT1, HIST3H3, CDK1, BLM, CCNE1, and UBE2C were significantly downregulated in patients with SCOS, compared with normal patients (Fig. 3A).