Although we did not evaluate the Nestin levels in our remaining or repopulated microglia, we found full repopulation of microglia 11–13 days after the withdrawal of PLX5622 in juvenile WT and prh mice and speculate that cortical repopulating microglia are proportional to the number of surviving immature microglia and respond to hydrocephalus. The gene discussed is NES; the disease is Hydrocephalus.