The JHH4 cells exhibited the relatively low mRNA and protein expression levels of SFXN1, suggesting a positive correlation between mRNA and protein expression of SFXN1. Because the HuH7 and JHH5 cells displayed the high SFXN1 expression level among liver cancer cell lines, SFXN1 knockout (SFXN1-KO) was generated in the two HCC cell lines using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system (Fig. 3C). This evidence concerns the gene SFXN1 and liver cancer.