Notably, in order to provide a non-proliferating tumor substrate, aimed exclusively to activate CAR-T cells and to avoid developing uncontrolled tumor burden, thus confounding the CRS symptoms and impacting mouse overall survival, humanized hGM-CSF/hIL3 NOG mice were engrafted with irradiated CD19+ Daudi (irrDaudi) FF-LUC cell lines by i.v. injection (Fig. 6a). This evidence concerns the gene CD19 and neoplasm.