Mechanical forces in tumor microenvironment may arise due to excessive growth of cells in a confinement as in case of solid tumors, interstitial flows within tumors and due to blood flow in the vasculature as in case of circulating tumor cells.[15] Thus, we tested whether mechanical force is responsible for the upregulation of IGFBP1 in confined cells. This evidence concerns the gene IGFBP1 and neoplasm.