To further confirm that IGFBP1 promotes tumor cell survival in confinement and subsequent metastasis by inhibiting SOD2 phosphorylation, we depleted IGFBP1 in SOD2‐depleted A549 cells rescued with rSOD2 WT or S27A (Figure S7A, Supporting Information) and found that IGFBP1 depletion dramatically increased the apoptosis and inhibited the migration of confined cells rescued with rSOD2 WT, while failed to do so in confined cells rescued with rSOD2 S27A (Figure7A,B). This evidence concerns the gene IGFBP1 and neoplasm.