To clarify whether secreted IGFBP1 is responsible for the migration of tumor cells, we neutralized IGFBP1 in culture medium with anti‐IGFBP1 antibody and examined the migration of A549 or SK‐Hep1 cells by using transwell migration assay, which showed that IGFBP1 neutralization dramatically attenuated the migration of these cells (Figure 2E). This evidence concerns the gene IGFBP1 and neoplasm.