In addition, we found the methylation level of cg05199874 was positively correlated with expression of multiple genes, including the signal peptide-CUB-EGF domain-containing protein 2 (SCUBE2), a novel tumor suppressor gene, which showed inhibitory roles in breast tumor invasion and migration through concerted activities with FOXA1 (35, 36). The gene discussed is SCUBE2; the disease is breast neoplasm.