CHRM1 and Alzheimer disease: This hypothesis was tested as a proof-of-concept by comparing the 2D BN-PAGE/SDS-PAGE-based SC/MC profile and IEF/SDS-PAGE-based PTMs (charged fractions) profile of the OXPHOS-associated proteins, specifically ATP5A and UQCRC2 in the post mortem hippocampus of a non-demented individual with relatively normal CHRM1 protein, to an AD patient with severely reduced hippocampal CHRM1 protein (Fig 4AB).