DCAF1 and melanoma: Further, stable depletion of VprBP with shRNA knockdown almost completely eliminated H2AT120p, and these changes could be rescued by ectopic expression of VprBP wild type but not VprBP K194R kinase-dead mutant in G361 and MeWo melanoma cells, as assessed by immunostaining and Western blot analyses (Fig. 1b, c and Additional file 1: Fig. S1a, b).