Therefore, to validate the significance of GLI3 genetic variation to pre-retinal neovascular disease, we performed an extension analysis of top-associated iROP SNPs, those with p ≤5×10−6 or greater significance levels, and SNPs regionally related for DR association within the Genetics of Latino Diabetic Retinopathy (GOLDR) GWAS dataset. This evidence concerns the gene GLI3 and diabetic retinopathy.