Finally, we identify additional SNPs with suggestive-level significance association with severe ROP disease, demonstrate cross-significance with previously identified ROP-associated SNPs, and further demonstrate relevance for GLI3 and these top SNPs within the human eye, finding expression in both human donor neurosensory retina as well as retinal pigment epithelium (RPE). Here, GLI3 is linked to retinopathy of prematurity.