Importantly, 2D proliferation, Matrigel invasiveness, chemotaxis, clonogenic survival and anchorage-free (anoikis) survival in human and mouse PTEN-negative PC cell lines were more dependent on AKT2 or AKT3 (the latter only in the case of LNCaP invasiveness) than on AKT1, consistent with a previous report [38] showing that 3D spheroid growth of PTEN-negative PC cells relies more on AKT2. This evidence concerns the gene AKT2 and pachyonychia congenita.