We reintroduced the 12 genes, CAV2, PHLDA1, DUSP23, VDAC3, DSG2, SPINT2, SPATS2L, IGFBP3, CD9, ALCAM, PRSS23, CDKN2A, constructed as Neoadjuvant Chemotherapy Score into the single cell sequencing data of LUAD and ESCC patients before and after neoadjuvant therapy as well as the CCLE database. The gene discussed is SPINT2; the disease is esophageal squamous cell carcinoma.