For the dominant bacteria in stage III-IV CRC,g__paraprevotelela.s__uncultured_organism was markedly relevant with multiple checkpoints (ADORA2A, VTCN1 and BTNL2 etc.)(as in Additional file 4: Fig. S4), immune-activating genes (ENTPD1, TNFSF18 and TNFRSF17 etc.)(as in Fig. 5B), immune-suppressive genes (KDR and LAG3 etc.)(as in Additional file 5: Fig. S5), chemokines (CCL11, ﻿CXCL12﻿ and CCL25 etc.)(Fig. 5D) and chemokine receptors (CCR2) (as in Additional file 6: Fig. S6 showed). Here, BTNL2 is linked to colorectal carcinoma.