To test the in vivo activity of CAR T cells responding to amph-FITC-coated tumours, we inoculated MSTO-211H tumours in NSG mice, adoptively transferred luciferase-expressing 4m5.3 or E2 CAR T cells with CD28 or 4-1BB co-stimulatory domains 7 days later, and then treated animals with repeated doses of i.t. amph-FITC (Fig. 7b). The gene discussed is CD28; the disease is neoplasm.