Our data confirmed that tumor-associated B cells mainly localized at the tumor margin and were observed infiltrating tumors in small clusters, and significant localization of IgD- (class-switched and IgM+) memory B cells and class-switched (IgG and IgA) antibody expression at the tumor site, which, depending on the isotype was positively or negatively associated with overall survival. The gene discussed is CD40LG; the disease is neoplasm.