To validate whether Tisp40 ameliorated cardiomyocyte injury through stimulating protein O-GlcNAcylation in vitro, sI/R-stimulated NRCMs with or without AdΔTM-HA infection were silenced with O-GlcNAc transferase (OGT) to block protein O-GlcNAcylation. The gene discussed is OGT; the disease is infection.