In addition, echocardiographic analysis also revealed profound cardiac hypertrophy in I/R-injured Tisp40 KO mice, as determined by the increased interventricular septal thickness at systole (IVSs) but not interventricular septal thickness at diastole (IVSd) (Fig. 3g and Supplementary Fig. 6G). This evidence concerns the gene CREB3L4 and cardiac hypertrophy.