Moreover, the arginine-deficient diet prevented the increase in NF-κB P65 subunit protein expression in TSC cyst-lining cells (Figures 7C, 7D, and S7D), which was associated with a decline in kidney mononuclear infiltration of Tsc1 KO kidneys, upon arginine-deficient diet treatment as was demonstrated by FACS analysis of F4/80+ cells in dissociated kidneys (Figures 7E and 7F). This evidence concerns the gene NFKB1 and cyst.