The islets was stimulated by 16.7 mM glucose in KRBH buffer, the secreted insulin was detected by ELISA assay; as shown in Fig 3B and 3C, overexpression of Rab26 significantly inhibited insulin secretion from islets at different detection time points compared with islets transduced with vector control, suggesting that overexpression of Rab26 suppresses insulin secretion not only in insulinoma cells, but also in freshly isolated islets, which is close to the in vivo physiological condition. The gene discussed is INS; the disease is pancreatic insulinoma.