REST directly bound to the promoter region of GCLM (–152 to –132, GCCGCAGGCCAAGGGCCAGTC) to repress its transcription, while knockdown of REST increased GCLM transcription and enhanced GCL enzymatic activity, leading to upregulation of GSH and GPX4 to reduce lipid peroxidation and ferroptosis in AKI. The gene discussed is REST; the disease is acute kidney injury.