ACTA1 and renal fibrosis: As anticipated, RestRTKO mice exhibited obvious attenuation of renal fibrosis, as evaluated by hematoxylin and eosin (H&E) staining, Masson’s staining, and immunohistochemical staining of α-SMA and fibronectin (Figure 8A), along with reduced Scr and BUN levels (Figure 8, B and C).