Moreover, oral gavage with 109 CFU/200 μL A. muciniphila reverses high fat obesity through improving intestinal barrier integrity, inflammation, energy balance, and triglyceride and glucose in the blood (57), which also suppresses mRNA expression of Tlr4 and Il-6 but not Tnf-α in adipose tissues (57). This evidence concerns the gene TNF and obesity due to melanocortin 4 receptor deficiency.