When comparing the absolute postprandial increase in plasma LEAP2 across this study [56], and the four analyses from three previously published studies [27, 28, 31] (overall two without obesity, three with obesity), there was a suggestion that the postprandial increase in plasma LEAP2 was greater with increasing energy intake at the meal, although with the small sample size this did not reach statistical significance (rs = + 0.70, P = .23) (Supplementary Fig. S2 [25]). This evidence concerns the gene LEAP2 and obesity due to melanocortin 4 receptor deficiency.