INS and obesity due to melanocortin 4 receptor deficiency: Plasma LEAP2 was therefore measured in a secondary analysis of our previous study comparing effects of exogenous and endogenous hyperghrelinemia on eating behavior [56], in which adults without obesity had visits after an overnight fast, or after consuming a breakfast meal without or with prior subcutaneous AG administration, with measurement of appetite ratings, food cue reactivity using a food picture evaluation fMRI task measuring BOLD signal from whole brain analysis, food intake at an ad libitum lunch meal, and plasma glucose, serum insulin, and triglycerides.