SPP1 and cancer: Our analysis revealed several macrophage subtypes which highly resembled those identified in previous single-cell studies were also detected in our study: Mac_SPP1 had an elevated angiogenic signature and an overall pro-tumor activity, as reported by studies in several other cancers [18, 19]; Mac_APOE and Mac_FABP5 showed increased expression of marker genes including APOE, FABP5, and TREM2, which were indicated in lipid-associated macrophages.