The genes included in “HCC-detect” and “HCC-spec” were significantly differentially methylated in a different validation set that had HCC cell free plasma DNA and tumor samples compared to cirrhosis and normal liver (GSE6377524) (Fig. 3A, B, D, E) except for GRID2IP which was significantly differentially methylated in HCC tissue samples (Fig. 3C) but didn’t reach significance in plasma because of the low number of reads in the serum sample (Fig. 3C). This evidence concerns the gene GRID2IP and neoplasm.