“HCC-detect” classifies cholangiocarcinoma cases as cancer, AUC of ICC (n = 39) against healthy blood (n = 968) is 0.9875 (sensitivity-97%; specificity-95%) (Supplementary Fig. 1A), however, “HCC-spec” does not differentiate ICC and blood with AUC 0.5436 (sensitivity-17%; specificity-95%) (Supplementary Fig. 1B) and is less sensitive in classifying “ICC from NAT, AUC of 0.833 (sensitivity-67%; and specificity-100%) (Supplementary Fig. 1A). This evidence concerns the gene BRD2 and intrahepatic cholangiocarcinoma.