Because the inflammatory response in treated mice was different than in control animals, the production of cytokines was evaluated by immunohistochemistry and automated morphometry, which showed more immune stained cells to IFN-γ, TNF-α, and IL-17 in the perivascular inflammatory infiltrate and granulomas of treated mice than in control animals (Figure 4C). The gene discussed is IFNG; the disease is Granuloma.