PIK3CA and colorectal carcinoma: Tan et al. (2021) pointed out that the PI3K-Akt signaling pathway is important in CRC, and found that promethazine promoted apoptosis by inhibiting the activation of PI3K-Akt signaling pathway in CRC cells. Next, we used molecular docking simulations to predict the binding capacity of AD-1 to PI3K and Akt. A lower binding energy indicates a more efficient and stable interaction between the compound and the receptor. As shown in Figures 4D, E, the binding energies of AD-1 to PI3K and Akt were −8.4 and −6.2 kcal/mol, respectively, indicating their good binding activities (Table 3).