Applying Akoya Bioscience OPAL 7-plex mIHC to the first cycle of NACT in the WSG-ADAPT-TN trial (n=66 TNBC patients), the increase in PD-1-+ CD4 and PD-1+ CD8 infiltration in both the tumor and in the stroma were significantly correlated with higher pCR (38). Here, CD4 is linked to neoplasm.