To further confirm the hypothesis that overexpression of miR-363 suppresses cell behavior by targeting E2F3 in CRC, siRNA-E2F3 was transfected to HCT-116 and SW480 cells, and the results indicated that knockdown of E2F3 dramatically inhibited the cell migration and invasion in HCT-116 and SW480 cells. The gene discussed is E2F3; the disease is colorectal carcinoma.