To investigate the role of CYP2E1 in glioma TME, we transfected CYP2E1 plasmids into HMC3 microglia and overexpression efficiency was verified by a fluorescence signal under an inverted microscope (Figure6A), RT‐qPCR (Figure 6B), and Western blotting (Figure 6C,D). The gene discussed is CYP2E1; the disease is glioma.