Our results revealed that miR-29a-3p-Robo1 signaling in HSCs mediated the pathogenesis of hepatic fibrosis, and overexpression of miR-29a-3p had a beneficial effect on schistosome-induced hepatic fibrosis by reducing the expression of Robo1 and preventing the activation of HSCs during infection. This evidence concerns the gene ROBO1 and Hepatic fibrosis.