Moreover, we further investigated hallmarks of cancer between two subclusters, that resulting in cluster 1 was associated with cell cycle and metabolism associated pathways, (Fig. 4B), and cluster 2 was associated with myogenesis, apical surface, KRAS signaling, TNFα signaling via NF-кB, inflammatory response, IL6-JAK/STAT3 signaling, UV response, epithelial-mesenchymal transition, the apical junction (Fig. 4B). The gene discussed is TNF; the disease is cancer.