ESR1 and breast carcinoma: Then, we detected the expression of DYNLT1 in different subtypes of breast cancer cell lines by RT-qPCR and western blot (WB) assay, respectively, and found that DYNLT1 was highly expressed in ER + breast cancer cells, including MCF-7 and T47D, and TNBC cells, including MDA-MB-468 and MDA-MB-231 (Fig. 1D and E).