To mimic a clinical situation of a tumor expressing both PD-L1 and PD-L2 for which a clinician could not decide which ICI was suitable, we reconstituted both hPD-L1- and hPD-L2-GPI proteins onto an SLB and imaged the behavior of hPD-1 on AND-TCR-expressing T cells. Here, CD274 is linked to neoplasm.