To evaluate these in vitro findings by murine in vivo experiments, we applied a tumor-bearing mice model in which we further introduced hPD-L1 into the OVA-transfected lymphoma cell line EL-4, E.G7 (Supplementary Fig. 5f), and transplanted E.G7-hPD-L1 into Rag2−/− albino C57BL/6 mice with Pdcd1−/− OT-I TCR-Tg CD8+ primary T cells reconstituted by hPD-1. This evidence concerns the gene CD8A and lymphoma.