In the present work, we performed a range of in vitro and in vivo experiments to investigate the important role of ferroptosis, a novel form of regulated cell death triggered by iron-dependent lipid peroxidation, in the pathogenesis of liver fibrosis in NASH with T2DM, and we further addressed whether AGER1 could improve liver fibrosis in NASH with T2DM by inhibiting ferroptosis. The gene discussed is DDOST; the disease is type 2 diabetes mellitus.