Surprisingly, we revealed that ANKRD29 expression notably correlated negatively with immune cells infiltration level using ESTIMATE algorithm analysis (a scoring method for tumor purity where the level of stromal cells and the infiltration level of immune cells in tumor tissues based on expression data present), which was consistent with the results that high ANKRD29 expression was positively related with different immune cells infiltration level by Single-sample gene set enrichment analysis (ssGSEA) in NSCLC (Fig. 6A, B). The gene discussed is ANKRD29; the disease is neoplasm.