ANKRD29 and non-small cell lung carcinoma: To validate the lower expression of ANKRD29 in NSCLC cells, qRT-PCR and western blot results revealed that ANKRD29 expression was decreased in NSCLC cancerous cell lines (A549, H1299, SPC-A1, H1650 and H1975), in which the normal human bronchial epithelial cell lines (16HBE) was the control cell line (Fig. 2H).