In addition, Kaplan-Meier survival curves proved that low expression levels of ANKRD29, ANKRD44 and ANKRD66 were associated with shorter overall survival compared to those with high expression levels in NSCLC patients (Fig. 2E), suggesting ANKRD29 could be more essential for NSCLC progression among these five hub genes, which was confirmed by mutation assays in cBioPortal websites and ROC analysis of TCGA-NSCLC datasets (Fig. S1A, B). The gene discussed is ANKRD29; the disease is non-small cell lung carcinoma.