NUCB2 and breast carcinoma: We also measured the levels of NUCB2/Nesfatin-1 in several breast cancer cell lines and an immortalized human mammary cell line, and found that both mRNA and protein were overexpressed in parallel in MDA-MB-231, BT-549, MDA-MB-468, and MCF-7 compared with MCF-10A, the non-cancer mammary epithelial cell line (Fig. 1D, E).