To determine whether HMGCR also upregulates cholesterol synthesis in breast cancer, we transiently transfected pcDNA3.1–6 × HisG-HMGCR plasmid into BT-549 and MDA-MB-231 cells and observed that overexpression of HMGCR increased cholesterol synthesis (Fig. 5A). The gene discussed is HMGCR; the disease is breast carcinoma.