However, as shown in Fig. 4A, there are still other signaling pathways involving NUCB2-related pathways such as cyclins and cell cycle regulation, Aryl Hydrocarbon receptor signaling, etc. Although our data indicate that NUCB2/Nesfatin-1 regulates cholesterol biosynthesis via the HMGCR/SREBP2 axis, further investigation is necessary to fully understand the role of NUCB2/Nesfatin-1 in breast cancer progression. This evidence concerns the gene HMGCR and breast cancer.