Then, FOXN2 expression in human HCC cell lines, including non-invasive Hep3B and HepG2, low-metastatic Huh7, Bel7402, HLE, HLF, and SMMC7721, metastatic HCC97H and HCC-LM3, and human hepatocyte QSG7701 and HL7702 cells was examined by WB. The gene discussed is FOXN2; the disease is hepatocellular carcinoma.